Wednesday, September 14, 2011

Making Proteins: Transcription and Translation

Transcription and Translation were probably the hardest subjects I have had to learn to I will do this section as organized as I can possibly make it.

Transcription: DNA-> RNA
In order for the DNA to make protein, it must first direct the synthesis of a complementary RNA molecule.
DNA can make RNA. RNA is synthesised according to the sequence of DNA bases. This is also known as genetic transcription. An enzyme called RNA polymerase breaks the hydrogen bonds between the DNA bases. Then RNA nucleotides that free float will reform hydrogen bonds with their complementary DNA bases. The RNA nucleotide containing adenine will bond to the DNA base thymine. The RNA nucleotide base uracil will bond to the DNA base adenine. In other words:
      • RNA adenine-> DNA thymine
      • RNA uracil-> DNA adenine
The ribose sugars and phosphates of th ribonucleotides join together to make a strand of RNA. when the strand of RNA is complete, it then detatches itself from the DNA then leaves the nucleus. Only one of the two of the strands from the DNAs double helix can be used to form a single strand of RNA.

There are three types of RNA: mRNA (messenger RNA), tRNA (transfer RNA) and rRNA (ribosomal RNA)
mRNA encodes structure for a particular protein. tRNA carries amine acids to ribosomes to add to a newly synthesized polypeptide. rRNA containts the structural components of ribosomes.

Translation is harder for me to provide with written words alone so here is a video I found on youtube that I show to the anatomy students I tutor. It helped them a lot! I donot own any rights to this video! This video is from youtube from a person I don't know but I am sharing it.







These notes were based off of actual notes from myself while I was in the class and further research outside the class. I also used the notes from my AWESOME professors who kindly handed them down to me and contributed extra information about this section. This information was also checked and clarified from various physiology books. For the pictures, I do not own any rights to them! If you have any questions feel free to ask on my blogspot or email me!


A deeper look in the Nucleus: DNA, Genes, and Cell Division

The nucleus is the brain of the cell. This is where all the magic happens. The nucleus is complex and I will describe it the best way I can. Inside the nucleus you will find many of these!

 This is a DNA :D

Let's start with the word chromotin. A chromotin is a long thread-like material that is actually a form that DNA takes withing  the nuclei of a nondividing cell. In other words, a chromotin is a piece of DNA with activated genes. A chromosome is the form that a chromotin takes when it is going to divide. This can easily be seen under a microscope.
 

A gene is a piece of DNA that contains information for an amino acid sequence to make proteins. In turn, it also can code for a trait.They are primary for making proteins. Genome is the total number of all genes in the body. There are about 25,000 different types of genes. Interesting enough, it has been recently discovered that a single gene can code for a number of different polypeptide chains. That first picture above is a piece of DNA that is considered to be a gene and can contain a code for different proteins. Proteome is the number of different proteins produced in the body. There are about 100,000 proteins in the body!! Wow! Despite the fact that these are some pretty high numbers scientists estimate that there are only 300 genes active in a given cell at a given time and the genes that are functioning in certain cells can be temporarily shut off and later turned back on.  Some genes can also be permenantly inactivated in each specialized cell type. This is important because different tissues and organs require that certain genes need to be turned off while others need to be permenantly turned on.

When a cell is going to divide, DNA must be copied prior to its cell division. This is called DNA replication. This can be a very confusing process so I will write it in steps. Refer to the picture above so you don't get lost!!
  1. An enzyme moves along the DNA breaking the hydrogen bonds between each pair bases of nucleotides. Its like the enzyme is a zipper and its unzipping the DNA along the hydrogen bonds.
  2. The now single strand of DNA is now floating withing the nucleus. Along with this strand are free floating nucleotides that then bind with the complementary nucleotides of the old strand.
      • Adenine-> Thymine
      • Guaine-> Cytocine
And there you have it, two new strands of DNA!
There are two types of cell division: Mitosis and Meiosis. For this session I will only talk about mitosis. I will have a different section dedicated to meiosis when I have a reproduction section. When a cell divides it has the exact same identity as the parent cell. This is when the chromotin convert themselves into chromosomes. I will put the steps of mitosis in number order along with a picture.
  1. Interphase- this is when the DNA replicate (not shown in picture but it IS there at the beginning so dont forget; cells have to replicate before dividing!)
  2. Prophase- the chromotin condense themselves into chromosomes; the duplicated chromotid on one side and the original on the other. They look like large "X"s. Also, the nuclear envelope fades away but don't worry it is still somewhat there!
  3. Metaphase- the chromosomes line up in the equator of the cell (the middle). An organelle called centrioles form spindle fibers that attatch to the center of the chromosome called the centromere.
  4. Anaphase- the spindle fibers then pull the duplicated chromosomes to opposite poles.
  5. Telophase- finally, the chromosomes are pulled apart so much that the cell cytoplasm breaks apart thus creating two new cells. The chromosomes turn back into chromotin and the nuclear envelope reappears concealing them.




These notes were based off of actual notes from myself while I was in the class and further research outside the class. I also used the notes from my AWESOME professors who kindly handed them down to me and contributed extra information about this section. This information was also checked and clarified from various physiology books. For the pictures, I do not own any rights to them! If you have any questions feel free to ask on my blogspot or email me!

Introduction to Cells

The world of biology revolves around cells! The cell is the basic unit of the body and every function that the pertains to the body. It is the smallest living subdivision we have in our bodies and is diverse in structure and function.Cells make up our tissues, organs,d and systems. Since this blog is about the physiology and not anatomy I will not go too deep into cells but I will go over the basics of cells that is important to know about. Some of the topics I will go into deeper detail but in a later blog.

Cells have tiny organs-like parts in them called organelles. Organelles provide structures and compartments in the cell. They also proide isolation of chemical reactions. An importing thing to remember about organelles are that each one performs a specific function.

The cell is made of three regions: the cell membrane, the cytoplasm, and the nucleus. The first I will go over is the cell membrane; also known as the plasma membrane.

Plasma Membrane
The plasma membrane is like the "shell" of the cell. It allows isolation and privacy and has its own cellular homeostasis pattern (refer to my homeostasis section on the main page). It seperates the intercellular environment from the extracellular environment. The plasma membrane is semipermeable, which means ot is in charge of maintaining everything that goes in and out of the cell.
The plasma membrane is made out of three structural components :lipids, proteins, and carbohydrates.
I do not own any rights to this picture!!

The segment with the orange balls with the little strings is called the phospholipid bilayer. The phosholipid bilayer is considered to be ampipathic, which means it loves water and hates it. The part that loves water is polar and the part that hates water is nonpolar. The orange balls are the polar regions that float in the intra/extracellular environment. The nonpolar region is the area with the little strings. These can also be refered to as nonpolar tails. The large various shaped beings that are protruding from the cell are proteins which I will go more into detail in another blog. The phospholipid bilayer is like the skin of the cell. Its what gives the cell its body. A cell without its phospholipid bilayer is like us without skin; we would be a bunch of organs walking around exposed to the elements.

Cytoplasm

The cytoplasm is basically everything inside the cell. It consists of cytosol, organelles, and the cytoskeleton. First, I will go over the cytosol. The cytosol is the jelly-like substance inside the cell. The aqueous substance is the location for many metabolic reactions and is also responsible for the storage of fat, glycogen, and secretory vessicles. It is the medium for diffusion and is the spot where protein synthesis occurs. It hold all the organelles and the cytoskeleton.
Next I will go over the cytoskeleton. The cytoskeleton is a network of protein fibers called microfilaments and microtubles located in the cytosol. it gives the cell structural support and shape. It also gives the cell external movement. Cells use cilia and flagella for movement. Sometimes cells can even have an amoeboid type movement that will be later discussed in another blog. Think of the cytoskeleton as being like our own skeleton! Our skeleton provides support, structure, movement, and protection for our bodies!
Now I shall go over the organelles! I will only go over a few that will be important. Remember: organelles are "organs".
Lysosome: It is a membrane bound organelle containing digestive enzymes. It can breakdown large molecules for nutrient purposes and destroy bacteria and old organelles. Think of the lysosome as the stomach of the cell. Since the lysosome contains all those digestive enzymes, breaking it would be bad. The enzymes would start digesting everything including the cell itself. This can be a good thing or a bad thing.
Mitochondria: It is a double membrane bound organelle with folds on the inside. Inner fold is called is called the cristae which surrounds the matrix, the space withing the cristae.

The mitochondria is the "powerhouse" of the cell. It is in charge of making the fuel for the cell called ATP (ATP  is very very very important so you gotta remember this!!!!) ATP is created by using the energy released from the breakdown of food. I will go more into detail about this process later. Mitochondria are unique because they posess their own DNA! All of the mitochondria in a person's body came from the mother's egg cell. Mitochondria is STRICTLY mother to child.
Ribosomes: Ribosomes free float of are attatched to the RER(rough endoplasmic reticulum). There is a large protein-RNA complex with ribosomes. Think of ribosomes as being a "protein factory". They synthesize proteins based on triplets of codons on a mRNA(dont worry I will have a blog specifically on this topic).
Rough Endoplasmic Reticulum (RER): The RER is coated with ribosomes which is what gives it that rough surface. This is just another work station for protein synthesis only any proteins created in the ribosomes are further modified in the golgi complex. It synthesizes proteins for the cell membrane (which are inserted into the membrane via vessicle), lysosomes, or to be exported outside of the cell.
Golgi Complex/Apparatus: They look like a flatten stack of pancakes! This folds that create the stacks are called cisternae. The Golgi Complex takes those those proteins made in the RER and completes them by modifying (posttransitional modifications), sorting them in a vessicle, then directing the finished products to their destinations.
Vessicle: Think of a vessicle being like mail.  It is a small membrane bound pod that it used to transport materials within the cell. It can carry proteins or nutrients to different parts of the cell. A vessicle can either fuse with the plasma membrane to secret wastes or bind with another organelle like a lysosome.
Smooth Endoplasmic Reticulum (SER): The SER contains no ribosomes and has a smooth surface. In it in charge of lipid synthesis, Calcuim storage, metabolizing various molecules, and the breakdown of carbohydrates.
Whew thats most of the important organelles! If there is a specific organelle you would like to know about that I didnt mention here just let me know.

Nucleus
The nucleus is like the brain of the cell; the control center. It contains information about protein synthesis. The nucleus is double membrane bound and along its surface are nuclear pores. An important thing to remember about the nucleus is that it contains chromotin. Inside the nucleus is another pod called the nucleolus! Inside the nucleolus is where RNA is synthesized.


Well thats basically the basics of cells. These notes were based off of actual notes from myself while I was in the class and further research outside the class. I also used the notes from my AWESOME professors who kindly handed them down to me and contributed extra information about this section. This information was also checked and clarified from various physiology books. For the pictures, I do not own any rights to them! If you have any questions feel free to ask on my blogspot or email me!